1. Recombinant Proteins
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Nucleocapsid Proteins
  5. SARS-CoV-2 N Protein C-terminal Domain
  6. Nucleocapsid Protein CTD domain, Virus (His)

Nucleocapsid Protein CTD domain, Virus (His)

Cat. No.: HY-P7439
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Nucleocapsid Protein CTD domain, Virus (His) is the C-terminal dimerisation domain (CTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein CTD domain has a stronger nucleic acid-binding activity than the NTD, which could be a nucleic acid-binding site .

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Description

Nucleocapsid Protein CTD domain, Virus (His) is the C-terminal dimerisation domain (CTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein CTD domain has a stronger nucleic acid-binding activity than the NTD, which could be a nucleic acid-binding site[1] [2].

Background

Coronaviruses are enveloped viruses with a positive-sense RNA genomeand and has a nucleocapsid of helical symmetry. The Nucleocapsid Protein localize to the cytoplasm and the nucleolus in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Nucleocapsid Protein is a basic protein and is abundant in coronaviruses, which has nonspecific binding activity toward nucleic acids, including ssRNA, ssDNA, and dsDNA[1].
SARS-CoV N protein contains two distinct RNA-binding domains (the N-terminal domain [NTD] and the CTD, they are linked by a poorly structured linkage region. The CTD spanning residues 248 to 365 shows stronger nucleic acid-binding activity than the NTD and the basic region between residues 248 to 280 of CTD forms a positively charged groove that represents a likely binding region for RNA. Additionally, crystal structures of CoV NPs-CTDs provide structural clues on how the building blocks for coronavirus nucleocapsid form through NP dimer[2].

Species

Virus

Source

E. coli

Tag

N-6*His

Accession

P0DTC9/QHD43423.2 (A119-Q239)

Gene ID

43740575  [NCBI]

Molecular Construction
N-term
6*His
Nucleocapsid CTD (A119-Q239)
Accession # P0DTC9/QHD43423.2
C-term
Synonyms
2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
AA Sequence

N/A

Molecular Weight

Approximately 16.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filter solution of PBS, 2 M Urea, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

Nucleocapsid Protein CTD domain, Virus (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nucleocapsid Protein CTD domain, Virus (His)
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HY-P7439
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