OPRM1 Protein, Human (Cell-Free, His)

The OPRM1 protein serves as a receptor for endogenous and synthetic opioids and undergoes conformational changes upon agonist binding, activating downstream signaling pathways. This includes coupling to G proteins, thereby regulating adenylyl cyclase, calcium channels, potassium channels, and intracellular signaling pathways. OPRM1 Protein, Human (Cell-Free, His) is the recombinant human-derived OPRM1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPRM1 Protein, Human (Cell-Free, His) is 400 a.a., with molecular weight of 47.6 kDa.

OPRM1 serves as a receptor for a range of endogenous opioids, including beta-endorphin and endomorphin, as well as responding to various natural and synthetic opioids like morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphine, and methadone. Upon agonist binding, the receptor undergoes conformational changes, leading to the activation of downstream signaling cascades. Agonist-induced activation involves coupling to an inactive GDP-bound heterotrimeric G-protein complex, initiating dissociation of the G-protein complex, and subsequent activation of G-protein alpha subunits, particularly the pertussis toxin-sensitive G(i) and G(o) G alpha proteins. This activation leads to a multitude of cellular responses, including the inhibition of adenylate cyclase activity, modulation of calcium channels, activation of potassium channels, and regulation of various intracellular signaling pathways such as MAPK, PLC, PKC, PI3K, and NF-kappa-B. Additionally, OPRM1 undergoes phosphorylation by GPRK subfamily protein kinases, and its association with beta-arrestins is crucial for short-term receptor desensitization, internalization through endocytosis, and subsequent recycling. The receptor, acting as a class A G-protein coupled receptor (GPCR), exhibits selective temporal coupling to G-proteins and undergoes rapid recycling, while its down-regulation pathways vary with the agonist and can occur dependent or independent of G-protein coupling. Furthermore, OPRM1 has the potential for heterooligomerization with other GPCRs, influencing agonist binding, signaling, and trafficking properties, ultimately playing a role in excitatory effects.

Human

E. coli Cell-free

N-10*His

P35372 (M1-P400)

4988

MDSSAAPTNASNCTDALAYSSCSPAPSPGSWVNLSHLDGNLSDPCGPNRTDLGGRDSLCPPTGSPSMITAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIINVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETAPLP

Monomer: 43 kDa Dimer: 100 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.