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  3. PADI2 Protein, Mouse (HEK293, N-His)

PADI2 Protein, Mouse (HEK293, N-His)

Cat. No.: HY-P78767A
SDS COA Handling Instructions

Padi2 Protein functions as an enzyme that catalyzes the deimination process, specifically targeting arginine residues within proteins. PADI2 Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived PADI2, expressed by HEK293 , with N-10*His labeled tag. ,

For research use only. We do not sell to patients.

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5 μg $115 In-stock
10 μg $195 In-stock
50 μg $550 In-stock
100 μg $880 In-stock
500 μg $2500 In-stock
1 mg $4000 In-stock
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Description

Padi2 Protein functions as an enzyme that catalyzes the deimination process, specifically targeting arginine residues within proteins. PADI2 Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived PADI2, expressed by HEK293 , with N-10*His labeled tag. ,

Background

The Padi2 protein is an enzyme involved in post-translational modification of proteins, specifically the conversion of arginine residues to citrulline. This modification has been implicated in a variety of biological processes, including gene regulation, immune responses, and inflammation.

Species

Mouse

Source

HEK293

Tag

N-10*His

Accession

Q08642 (M1-P673)

Gene ID

18600

Synonyms
PADI2; PAD-H19; KIAA0994; PAD2; PDI2
AA Sequence

MQPPIRENMLRERTVRLQYGSRVEAVYVLGTQLWTDVYSAAPAGAKTFSLKHSEGVKVEVVRDGEAEEVVTNGKQRWALSPSSTLRLSMAQASTEASSDKVTVNYYEEEGSAPIDQAGLFLTAIEISLDVDADRDGEVEKNNPKKASWTWGPEGQGAILLVNCDRDTPWLPKEDCSDEKVYSKQDLQDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRQKLYHVVKYTGGSAELLFFVEGLCFPDESFSGLVSIHVSLLEYMAEGIPLTPIFTDTVMFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYMNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPIKQLLGPDFGYVTREPLFETVTSLDSFGNLEVSPPVTVNGKEYPLGRILIGSSFPLSGGRRMTKVVRDFLQAQQVQAPVELYSDWLTVGHVDEFMTFIPIPGKKEFRLLMASTSACYQLFREKQKAGHGEAVMFKGLGGMSSKRITINKILSNESLTQENQYFQRCLDWNRDILKRELALTEKDIIDLPALFKMDENHQARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLETHVRGLLEPLGLACTFIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP

Molecular Weight

Approximately 80-85 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 1mM EDTA, 0.5 mM TCEP, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PADI2 Protein, Mouse (HEK293, N-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PADI2 Protein, Mouse (HEK293, N-His)
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HY-P78767A
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