PARK7/DJ-1 Protein, Human (His)

The multifunctional PARK7/DJ-1 protein plays a key role in cellular defense against oxidative stress and cell death. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease, and participates in neuroprotective mechanisms by stabilizing NFE2L2 and PINK1 proteins. PARK7/DJ-1 Protein, Human (His) is the recombinant human-derived PARK7/DJ-1 protein, expressed by E. coli , with C-His labeled tag. The total length of PARK7/DJ-1 Protein, Human (His) is 189 a.a., with molecular weight of ~23-25 kDa.

PARK7/DJ-1, a multifunctional protein, assumes a pivotal role in cellular defense against oxidative stress and cell death, functioning as an oxidative stress sensor, redox-sensitive chaperone, and protease. It engages in neuroprotective mechanisms, stabilizing NFE2L2 and PINK1 proteins, positively regulating the androgen signaling pathway for male fertility, and modulating cell growth and transformation through its impact on the NF-kappa-B signaling pathway. Despite its disputed role as a protein and nucleotide deglycase, implicated in repairing glycated proteins and nucleotides, PARK7/DJ-1's involvement in a nucleotide repair system named guanine glycation repair (GG repair) is recognized, mitigating damage caused by methylglyoxal and glyoxal. Acting on early glycation intermediates, it prevents the formation of advanced glycation endproducts (AGE) that inflict irreversible damage. Beyond its role in glycation repair, PARK7/DJ-1 maintains mitochondrial morphology and function, facilitates autophagy of dysfunctional mitochondria, regulates expression or stability of mitochondrial uncoupling proteins, and orchestrates protective responses in pancreatic islets. Moreover, it exerts control over inflammatory responses in astrocytes, modulates endocytosis, binds to specific mRNAs, and exhibits metal-binding capabilities, enhancing cellular defense mechanisms against metal toxicity.

Measured by its ability to cleave the substrate 20μM phenylglyoxal that incubate at room temperature in kinetic mode for 5minutes.The specific activity is >400 pmol/min/µg.

Human

E. coli

C-His

Q99497 (M1-D189)

11315  [NCBI]

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD

Approximately 23-25 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 3 mM DTT, 5% glycerol, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.