PARP1 Protein, Human (sf9, His)

The PARP1 protein is a polyADP-ribosyltransferase that crucially mediates polyADP-ribosylation (PARsylation) in DNA repair, including base excision and double-strand breaks. By interacting with factors such as HPF1 and NMNAT1, the specificity of PARP1 extends to various amino acids. PARP1 Protein, Human (sf9, His) is the recombinant human-derived PARP1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of PARP1 Protein, Human (sf9, His) is 1014 a.a., with molecular weight of 100-110 kDa.

PARP1, a poly-ADP-ribosyltransferase, is a crucial mediator of poly-ADP-ribosylation (PARsylation) of proteins, playing a pivotal role in DNA repair, including base excision repair and double-strand breaks repair. Specificity for various amino acids is conferred by interacting factors like HPF1 and NMNAT1. In the context of DNA damage, PARP1 recognizes and binds DNA breaks, recruiting HPF1 and initiating serine ADP-ribosylation of target proteins such as histones. This PARsylation facilitates chromatin decompaction, recruitment of repair factors, and ultimately DNA strand break repair. Additionally, PARP1 is implicated in transcription regulation, acting both as a positive and negative regulator of transcription elongation. It also contributes to processes like replication fork progression, parthanatos, negative regulation of the cGAS-STING pathway, and inhibition of adipogenesis. Furthermore, PARP1 generates nuclear ATP in collaboration with NMNAT1, PARG, and NUDT5, promoting extensive chromatin remodeling. In apoptotic contexts, cleaved and translocated PARP1 serves as a poly-ADP-ribose carrier, inducing AIFM1-mediated apoptosis. The multifaceted functions of PARP1 highlight its integral role in diverse cellular processes.

Measured by its binding ability in a functional ELISA. Immobilized human PARP-His at 2 μg/mL (100 μL/well) can bind human HSP70-His and the EC₅₀ is 0.2-1.0 μg/mL.

Human

Sf9 insect cells

C-His

P09874 (M1-W1014)

142  [NCBI]

MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

100-110 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10 % Glycerol, pH 7.5, 2 mM EDTA, 0.5 mM TCEP.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.