1. Recombinant Proteins
  2. Others
  3. PARP3 Protein, Human (His, Strep)

PARP3 Protein, Human (His, Strep)

Cat. No.: HY-P702866
Handling Instructions

The PARP3 protein is a mono-ADP ribosyltransferase that responds to DNA damage, focusing on mono-ADP ribosylation of proteins such as histone H2B, XRCC5, and XRCC6. PARP3 plays a key role in the DNA repair process, participating in precise nonhomologous end joining and inhibiting G-quadruplex structure. PARP3 Protein, Human (His, Strep) is the recombinant human-derived PARP3 protein, expressed by E. coli , with N-His, N-Strep II labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The PARP3 protein is a mono-ADP ribosyltransferase that responds to DNA damage, focusing on mono-ADP ribosylation of proteins such as histone H2B, XRCC5, and XRCC6. PARP3 plays a key role in the DNA repair process, participating in precise nonhomologous end joining and inhibiting G-quadruplex structure. PARP3 Protein, Human (His, Strep) is the recombinant human-derived PARP3 protein, expressed by E. coli , with N-His, N-Strep II labeled tag.

Background

PARP3 protein, a mono-ADP-ribosyltransferase, plays a pivotal role in the cellular response to DNA damage, as highlighted in various studies reported in PubMed. Unlike PARP1 and PARP2, PARP3 specifically mediates mono-ADP-ribosylation, targeting glutamate, aspartate, or lysine residues on select proteins, such as histone H2B, XRCC5, and XRCC6, involved in chromatin architecture and DNA metabolism. This protein is instrumental in DNA repair processes, participating in the detection and signaling pathway triggered by DNA strand breaks. In single-strand break repair, it catalyzes mono-ADP-ribosylation of histone H2B, fostering accurate non-homologous end-joining (NHEJ) in collaboration with the XRCC5-XRCC6 heterodimer. PARP3 also suppresses G-quadruplex structures and is associated with various DNA repair factors, contributing to the cellular response to both exogenous and endogenous DNA damage. Additionally, it acts as a negative regulator of immunoglobulin class switch recombination and is capable of ADP-ribosylating DNA strand break termini, showcasing its multifaceted involvement in genomic maintenance and cellular processes.

Species

Human

Source

E. coli

Tag

N-His;N-Strep

Accession

Q9Y6F1-1 (M1-L533)

Gene ID

10039

Molecular Construction
N-term
His-Strep II
PARP3 (M1-L533)
Accession # Q9Y6F1-1
C-term
Synonyms
Protein mono-ADP-ribosyltransferase PARP3; ARTD3; IRT1; ADPRT-3; PARP-3
Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PARP3 Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PARP3 Protein, Human (His, Strep)
Cat. No.:
HY-P702866
Quantity:
MCE Japan Authorized Agent: