PARP3 Protein, Human (His, StrepⅡ)

The PARP3 protein is a mono-ADP ribosyltransferase that responds to DNA damage, focusing on mono-ADP ribosylation of proteins such as histone H2B, XRCC5, and XRCC6. PARP3 plays a key role in the DNA repair process, participating in precise nonhomologous end joining and inhibiting G-quadruplex structure. PARP3 Protein, Human (His, Strep) is the recombinant human-derived PARP3 protein, expressed by E. coli , with N-His, N-Strep II labeled tag.

PARP3 protein, a mono-ADP-ribosyltransferase, plays a pivotal role in the cellular response to DNA damage, as highlighted in various studies reported in PubMed. Unlike PARP1 and PARP2, PARP3 specifically mediates mono-ADP-ribosylation, targeting glutamate, aspartate, or lysine residues on select proteins, such as histone H2B, XRCC5, and XRCC6, involved in chromatin architecture and DNA metabolism. This protein is instrumental in DNA repair processes, participating in the detection and signaling pathway triggered by DNA strand breaks. In single-strand break repair, it catalyzes mono-ADP-ribosylation of histone H2B, fostering accurate non-homologous end-joining (NHEJ) in collaboration with the XRCC5-XRCC6 heterodimer. PARP3 also suppresses G-quadruplex structures and is associated with various DNA repair factors, contributing to the cellular response to both exogenous and endogenous DNA damage. Additionally, it acts as a negative regulator of immunoglobulin class switch recombination and is capable of ADP-ribosylating DNA strand break termini, showcasing its multifaceted involvement in genomic maintenance and cellular processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-StrepⅡ;N-His

Q9Y6F1-1 (M1-L533)

10039

Approximately 63.3 KDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.