1. Recombinant Proteins
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins
  4. B7-DC/PD-L2/CD273 B7-DC/PD-L2/CD273 B7-DC/PD-L2/CD273
  5. PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His)

PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His)

Cat. No.: HY-P72493
COA Handling Instructions

PDCD1LG2 is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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50 μg $235 In-stock
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Description

PDCD1LG2 is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Programmed cell death 1 ligand 2 is a cell surface receptor also known as PD-L2, B7-DC or CD273. PDCD1LG2 is an immune checkpoint receptor ligand that plays a negative regulatory role in adaptive immune response. PDCD1LG2 binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 plays an important role in immune tolerance and autoimmunity, and both PD-L1 and PDCD1LG2 can inhibit T cell proliferation and inflammatory cytokine production. Blocking PDCD1LG2 exacerbates experimental autoimmune encephalomyelitis. PDCD1LG2 triggers IL-12 production in mouse dendritic cells, leading to T-cell activation. Treatment with PDCD1LG2 Ig led to a proliferation of T helper cells. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T cell-mediated immune responses and can be used as a biomarker or prognostic indicator[1][2][3][4][5].

Species

Rhesus Macaque; Cynomolgus

Source

HEK293

Tag

C-6*His

Accession

A4GW30 (L20-P219)

Gene ID
Molecular Construction
N-term
PD-L2 (L20-P219)
Accession # A4GW30
6*His
C-term
Synonyms
Programmed cell death 1 ligand 2; Pdcd1lg2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273
AA Sequence

LFTVTVPKELYIIEHGSNATLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLSLGKALFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNISVPANTSHSRTPEGLYQVTSVLRLKPHPGRNFSCVFWNAQVRELTLASIDLQSQIEPRTHP

Molecular Weight

30-40 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His)
Cat. No.:
HY-P72493
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