PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His)

PDCD1LG2 is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Rhesus macaque/Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-6*His labeled tag.

Programmed cell death 1 ligand 2 is a cell surface receptor also known as PD-L2, B7-DC or CD273. PDCD1LG2 is an immune checkpoint receptor ligand that plays a negative regulatory role in adaptive immune response. PDCD1LG2 binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 plays an important role in immune tolerance and autoimmunity, and both PD-L1 and PDCD1LG2 can inhibit T cell proliferation and inflammatory cytokine production. Blocking PDCD1LG2 exacerbates experimental autoimmune encephalomyelitis. PDCD1LG2 triggers IL-12 production in mouse dendritic cells, leading to T-cell activation. Treatment with PDCD1LG2 Ig led to a proliferation of T helper cells. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T cell-mediated immune responses and can be used as a biomarker or prognostic indicator^{[1][2][3][4][5]}.

Rhesus Macaque; Cynomolgus

HEK293

C-6*His

A4GW30 (L20-P219)

716003  [NCBI]

LFTVTVPKELYIIEHGSNATLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLSLGKALFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNISVPANTSHSRTPEGLYQVTSVLRLKPHPGRNFSCVFWNAQVRELTLASIDLQSQIEPRTHP

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.