PDGF-DD Protein, Mouse (His-SUMO)

PDGF-DD protein is an important growth factor that controls embryonic development, cell proliferation, migration, survival and chemotaxis, and has a potent mitogenic effect on mesenchymal cells. It plays a key role in wound healing and has carcinogenic potential, contributing to tumor formation. PDGF-DD Protein, Mouse (His-SUMO) is the recombinant mouse-derived PDGF-DD protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

PDGF-DD Protein emerges as a crucial growth factor governing embryonic development, cell proliferation, migration, survival, and chemotaxis. Renowned for its potent mitogenic effects on mesenchymal cells, PDGF-DD assumes a pivotal role in wound healing and, intriguingly, harbors oncogenic potential capable of inducing tumor formation. Its influence extends to the intricate orchestration of events during angiogenesis, promoting macrophage recruitment, heightened interstitial pressure, and blood vessel maturation. PDGF-DD's involvement is notable in the initiation of processes leading to mesangial proliferative glomerulonephritis, marked by monocyte and macrophage influx, as well as extracellular matrix production. Structurally, PDGF-DD exists as a homodimer linked by disulfide bonds, engaging in interactions with PDGFRB homodimers and heterodimers formed by PDGFRA and PDGFRB, contributing to its versatile regulatory mechanisms.

Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×10⁴ units/mg.

Mouse

E. coli

N-His;N-SUMO

Q925I7-1 (T24-R370)

71785  [NCBI]

TPQRASIKALRNANLRRDESNHLTDLYQREENIQVTSNGHVQSPRFPNSYPRNLLLTWWLRSQEKTRIQLSFDHQFGLEEAENDICRYDFVEVEEVSESSTVVRGRWCGHKEIPPRITSRTNQIKITFKSDDYFVAKPGFKIYYSFVEDFQPEAASETNWESVTSSFSGVSYHSPSITDPTLTADALDKTVAEFDTVEDLLKHFNPVSWQDDLENLYLDTPHYRGRSYHDRKSKVDLDRLNDDVKRYSCTPRNHSVNLREELKLTNAVFFPRCLLVQRCGGNCGCGTVNWKSCTCSSGKTVKKYHEVLKFEPGHFKRRGKAKNMALVDIQLDHHERCDCICSSRPPR

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.