PDGF-DD Protein, Human (HEK293, His)

The PDGF-DD protein is a key growth factor with critical regulatory effects on embryonic development, cell proliferation, migration, survival, and chemotaxis. It is a potent mitogen for mesenchymal cells and plays a crucial role in wound healing. PDGF-DD Protein, Human (HEK293, His) is the recombinant cynomolgus-derived PDGF-DD protein, expressed by HEK293 , with N-His labeled tag.

PDGF-DD protein functions as a crucial growth factor with diverse roles in embryonic development, cell proliferation, migration, survival, and chemotaxis. It serves as a potent mitogen for mesenchymal cells and plays a pivotal role in wound healing processes. Moreover, PDGF-DD is involved in orchestrating events critical for angiogenesis, including macrophage recruitment, increased interstitial pressure, and blood vessel maturation. Its ability to initiate processes leading to mesangial proliferative glomerulonephritis, characterized by monocyte and macrophage influx along with extracellular matrix production, underscores its regulatory impact in pathophysiological conditions. The protein forms homodimers through disulfide linkages and interacts with PDGFRB homodimers, as well as heterodimers formed by PDGFRA and PDGFRB, illustrating its complex engagement in signaling pathways.

Human

HEK293

N-His

Q9GZP0-1 (R19-R370)

80310

50-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.