PDK4 Protein, Human (GST)

PDK4 Protein phosphorylates PDHA1 and PDHA2, inhibiting pyruvate dehydrogenase activity. This reduces glucose utilization, enhances fat metabolism, and helps maintain blood glucose levels during fasting. PDK4 also regulates fatty acid oxidation and biosynthesis, generates reactive oxygen species, protects epithelial cells, and regulates cell proliferation through metabolic regulation. PDK4 Protein, Human (GST) is the recombinant human-derived PDK4 protein, expressed by E. coli , with N-GST labeled tag. The total length of PDK4 Protein, Human (GST) is 411 a.a., .

PDK4, a pivotal kinase in the intricate orchestration of glucose and fatty acid metabolism, exerts its regulatory influence through the phosphorylation of pyruvate dehydrogenase subunits PDHA1 and PDHA2. By inhibiting pyruvate dehydrogenase activity, PDK4 finely tunes metabolite flux within the tricarboxylic acid cycle, dampens aerobic respiration, and curtails the conversion of pyruvate into acetyl-coenzyme A. This orchestrated inhibition, especially during prolonged fasting and starvation, reduces glucose utilization and enhances fat metabolism, crucial for energy homeostasis. Notably, PDK4 plays a crucial role in maintaining normal blood glucose levels under conditions of nutrient scarcity and is intricately involved in the insulin signaling cascade. Its modulation of pyruvate dehydrogenase activity not only helps regulate blood pH but also prevents the accumulation of ketone bodies during starvation. In the fed state, PDK4 mediates cellular responses to varying glucose levels and high-fat diets, acting as a key regulator of both fatty acid oxidation and de novo fatty acid biosynthesis. Furthermore, PDK4 contributes to the generation of reactive oxygen species, protects detached epithelial cells against anoikis, and participates in cell proliferation through its role in regulating carbohydrate and fatty acid metabolism.

Human

E. coli

N-GST

Q16654 (M1-M411)

5166

MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM

Approximately 72.9 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT, 0.1 M Trehalose.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.