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PDYN Protein, Human (HEK293, His)

Cat. No.: HY-P71058
COA Handling Instructions

The PDYN protein, or prodynorphin, regulates Leu-enkephalins, mimicking opiate drug effects and impacting pain perception and stress responses. Dynorphin peptides, like Dynorphin A(1-13), uniquely regulate the kappa opioid receptor, with A(1-13) being exceptionally potent—700 times stronger than Leu-enkephalin—displaying typical opioid activity. PDYN Protein, Human (HEK293, His) is the recombinant human-derived PDYN protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDYN Protein, Human (HEK293, His) is 234 a.a., with molecular weight of ~35.0 kDa.

For research use only. We do not sell to patients.

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10 μg $150 In-stock
50 μg $420 In-stock
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Description

The PDYN protein, or prodynorphin, regulates Leu-enkephalins, mimicking opiate drug effects and impacting pain perception and stress responses. Dynorphin peptides, like Dynorphin A(1-13), uniquely regulate the kappa opioid receptor, with A(1-13) being exceptionally potent—700 times stronger than Leu-enkephalin—displaying typical opioid activity. PDYN Protein, Human (HEK293, His) is the recombinant human-derived PDYN protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDYN Protein, Human (HEK293, His) is 234 a.a., with molecular weight of ~35.0 kDa.

Background

The PDYN protein, also known as prodynorphin, is associated with Leu-enkephalins, which compete with and emulate the effects of opiate drugs. These peptides play a pivotal role in various physiological functions, including pain perception and responses to stress. Additionally, dynorphin peptides, such as Dynorphin A(1-13), exhibit differential regulation of the kappa opioid receptor. Notably, Dynorphin A(1-13) demonstrates typical opioid activity and is remarkably more potent, being 700 times stronger than Leu-enkephalin.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P01213 (D21-A254)

Gene ID
Molecular Construction
N-term
PDYN (D21-A254)
Accession # P01213
6*His
C-term
Synonyms
ADCA; PENKB; SCA23; PDYN; Dynorphin B; Big Dyn
AA Sequence

DCLSRCSLCAVKTQDGPKPINPLICSLQCQAALLPSEEWERCQSFLSFFTPSTLGLNDKEDLGSKSVGEGPYSELAKLSGSFLKELEKSKFLPSISTKENTLSKSLEEKLRGLSDGFREGAESELMRDAQLNDGAMETGTLYLAEEDPKEQVKRYGGFLRKYPKRSSEVAGEGDGDSMGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDPNAYSGELFDA

Molecular Weight

Approximately 35.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PDYN Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PDYN Protein, Human (HEK293, His)
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HY-P71058
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