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  3. Pepsinogen C/PGC Protein, Rat (HEK293, His)

Pepsinogen C (PGC) functions as an enzyme with the ability to hydrolyze a diverse range of proteins.Pepsinogen C/PGC Protein, Rat (HEK293, His) is the recombinant rat-derived Pepsinogen C/PGC protein, expressed by HEK293 , with C-His labeled tag.

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  • Biological Activity

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Description

Pepsinogen C (PGC) functions as an enzyme with the ability to hydrolyze a diverse range of proteins.Pepsinogen C/PGC Protein, Rat (HEK293, His) is the recombinant rat-derived Pepsinogen C/PGC protein, expressed by HEK293 , with C-His labeled tag.

Background

Pepsinogen C (PGC) is an enzyme that functions as a precursor to the digestive enzyme pepsin. PGC possesses the ability to hydrolyze a diverse range of proteins. This enzyme is primarily synthesized and secreted by the gastric chief cells in the stomach lining. Once released into the acidic environment of the stomach, PGC undergoes cleavage to form pepsin, an active protease responsible for breaking down dietary proteins into smaller peptides. The conversion of PGC to pepsin is facilitated by the low pH of the stomach, triggering the activation of this zymogen. The role of PGC in the digestive process is crucial for the initial breakdown of ingested proteins, enabling their subsequent digestion and absorption in the gastrointestinal tract. Understanding the activation and function of PGC contributes to insights into the intricate mechanisms of protein digestion and gastric physiology.

Biological Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is 41133.212 pmol/min/μg that incubate at room temperature for 10 minutes.

Species

Rat

Source

HEK293

Tag

C-6*His

Accession

P04073 (S17-V392)

Gene ID
Molecular Construction
N-term
PGC (S17-V392)
Accession # P04073
His
C-term
Synonyms
Gastricsin; Pepsinogen C; PGC; PEPC
AA Sequence

SLLRVPLRKMKSIRETMKEQGVLKDFLKTHKYDPGQKYHFGNFGDYSVLYEPMAYMDASYFGEISIGTPPQNFLVLFDTGSSNLWVSSVYCQSEACTTHARFNPSKSSTYYTEGQTFSLQYGTGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPGLSSGGATTALQGMLGEGALSQPLFGVYLGSQQGSNGGQIVFGGVDKNLYTGEITWVPVTQELYWQITIDDFLIGDQASGWCSSQGCQGIVDTGTSLLVMPAQYLSELLQTIGAQEGEYGEYFVSCDSVSSLPTLSFVLNGVQFPLSPSSYIIQEDNFCMVGLESISLTSESGQPLWILGDVFLRSYYAIFDMGNNKVGLATSV

Molecular Weight

Approximately 42 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Pepsinogen C/PGC Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Pepsinogen C/PGC Protein, Rat (HEK293, His)
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