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Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human (HEK293, His, Avi)

Cat. No.: HY-P702840
COA Handling Instructions

HLA-A*0201 MAGE-A4 Complex Protein, a member of the major histocompatibility complex (MHC) class I family, is highlighted. Additionally, the Chimeric HLA-A*0201 WT-1 Complex Tetramer is recognized within the MHC class I family. Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human (HEK293, His, Avi) is the recombinant human-derived HLA-A*02:01&B2M Monomer, expressed by HEK293 , with C-His, C-Avi labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $160 In-stock
50 μg $300 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

HLA-A*0201 MAGE-A4 Complex Protein, a member of the major histocompatibility complex (MHC) class I family, is highlighted. Additionally, the Chimeric HLA-A*0201 WT-1 Complex Tetramer is recognized within the MHC class I family. Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human (HEK293, His, Avi) is the recombinant human-derived HLA-A*02:01&B2M Monomer, expressed by HEK293 , with C-His, C-Avi labeled tag. ,

Background

The Chimeric HLA-A*0201 WT-1 Complex belongs to the major histocompatibility complex (MHC) class I family. The Chimeric HLA-A*0201 WT-1 Complex Tetramer is also a member of the MHC class I family.

Biological Activity

Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1 μg/mL (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of ≤10 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-His;C-Avi

Accession

A0A140T913 (G25-T305)&P61769 (I21-M119)

Gene ID

/&567

Synonyms
MHC; HLA-A*02:01; Peptide Ready
Molecular Weight

50-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human (HEK293, His, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Peptide Ready HLA-A*02:01&B2M Monomer Protein, Human (HEK293, His, Avi)
Cat. No.:
HY-P702840
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