Periostin Protein, Mouse (Q62009-2, HEK293, His)

Periostin protein is a multifunctional molecule that plays a key role in cellular processes by inducing cell attachment and spreading, emphasizing its importance in cell adhesion dynamics. Furthermore, Periostin contributes to the structural integrity of connective tissue by enhancing the binding of BMP1 to the fibronectin matrix and promoting subsequent proteolytic activation of lysyl oxidase LOX. Periostin Protein, Mouse (Q62009-2, HEK293, His) is the recombinant mouse-derived Periostin protein, expressed by HEK293 , with C-His labeled tag.

Periostin protein, a multifunctional molecule, plays a pivotal role in cellular processes by inducing cell attachment and spreading, demonstrating its significance in cell adhesion dynamics. Moreover, Periostin contributes to the structural integrity of connective tissues by enhancing the incorporation of BMP1 into the fibronectin matrix, leading to the subsequent proteolytic activation of lysyl oxidase LOX. Structurally, Periostin exists as a homodimer, and functionally, it interacts with key partners including BMP1 and fibronectin. This intricate network of interactions underscores the diverse functions of Periostin in mediating cellular adhesion and contributing to the extracellular matrix architecture of connective tissues, highlighting its essential role in maintaining tissue integrity and function.

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10 μg/mL, 100 μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.

Mouse

HEK293

C-His

Q62009-2/NP_056599.1 (N24-Q811)

50706  [NCBI]

90-95 kDa

Greater than 90% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.