pfHPRT Protein, P. falciparum (His)

The pfHPRT (Plasmodium falciparum hypoxanthine-guanine phosphoribosyltransferase) protein is a member of the LDH/MDH (lactate dehydrogenase/malate dehydrogenase) superfamily. It plays a crucial role in the purine metabolism salvage pathway of the malaria-causing parasite Plasmodium falciparum. pfHPRT Protein, P. falciparum (His) is the recombinant pfHPRT protein, expressed by E. coli , with N-His labeled tag. The total length of pfHPRT Protein, P. falciparum (His) is 231 a.a., with molecular weight of ~28.6 kDa.

pfHPRT (Plasmodium falciparum hypoxanthine-guanine phosphoribosyltransferase) protein is a member of the LDH/MDH (lactate dehydrogenase/malate dehydrogenase) superfamily. It plays a crucial role in the salvage pathway of purine metabolism in the malaria-causing parasite Plasmodium falciparum. pfHPRT catalyzes the conversion of hypoxanthine and guanine to their respective nucleotide forms, in the presence of phosphoribosyl pyrophosphate (PRPP). This enzymatic activity is essential for the parasite's survival, as it provides a means to generate purine nucleotides required for DNA and RNA synthesis. Due to its involvement in purine salvage, pfHPRT has been explored as a potential target for antimalarial drug development. Inhibition of pfHPRT could disrupt the parasite's purine metabolism, leading to the depletion of nucleotides and subsequent parasite death. Understanding the structure, function, and regulation of pfHPRT may contribute to the development of novel strategies to combat malaria.

Others

E. coli

N-His

Q76NM3 (M1-L231)

814112  [NCBI]

Approximately 28.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.