PFKM Protein, Human (sf9, His-GST)

PFKM protein, a key regulator in cellular metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This fundamental enzymatic step controls glucose entry into glycolysis, influencing downstream energy production and cellular metabolism. PFKM's pivotal role highlights its significance in orchestrating metabolic flux and maintaining cellular energy balance. PFKM Protein, Human (sf9, His-GST) is the recombinant human-derived PFKM protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The PFKM protein assumes a pivotal role in cellular metabolism by catalyzing the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP as a substrate. This enzymatic activity marks the initial and committing step of glycolysis, a fundamental metabolic pathway central to energy production. By facilitating the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, PFKM plays a key role in regulating the entry of glucose into the glycolytic pathway, thereby influencing downstream energy production and cellular metabolic processes. The significance of PFKM in catalyzing this essential reaction underscores its importance in orchestrating the metabolic flux and maintaining cellular energy balance.

Human

Sf9 insect cells

N-His;N-GST

P08237-1 (T2-V780)

5213  [NCBI]

Approximately 113 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.