PFKP Protein, Human (sf9, His-GST)

PFKP protein, central to cellular energy metabolism, catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, initiating glycolysis. This key enzymatic step regulates glucose entry into glycolysis, initiating essential biochemical reactions for ATP and metabolite generation. PFKP's importance in orchestrating this process highlights its central role in cellular energy homeostasis and broader metabolic regulation. PFKP Protein, Human (sf9, His-GST) is the recombinant human-derived PFKP protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag. The total length of PFKP Protein, Human (sf9, His-GST) is 784 a.a., with molecular weight of ~113.4 kDa.

The PFKP protein plays a pivotal role in cellular energy metabolism by catalyzing the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP as a substrate. This enzymatic activity marks the initial and committing step of glycolysis, a fundamental pathway crucial for energy production. Through its catalytic function, PFKP regulates the entry of glucose into glycolysis, initiating a series of biochemical reactions that contribute to the generation of ATP and other essential metabolites. The importance of PFKP in orchestrating this key step underscores its central role in cellular energy homeostasis, emphasizing its significance in the broader context of metabolic regulation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-His;N-GST

Q01813 (M1-V784)

5214  [NCBI]

Approximately 113.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 8.0, 0.5 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.