PGK1 Protein, Human (HEK293, His)

The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PGK1 Protein, Human (HEK293, His) is 416 a.a., with molecular weight of 46-50 kDa.

Phosphoglycerate kinase 1 (PGK1) plays a crucial role in glycolysis by catalyzing the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, contributing to the production of ATP. Beyond its established role in glycolytic energy metabolism, PGK1 is suggested to function as a cofactor protein, particularly in association with polymerase alpha, where it acts as a primer recognition protein. Additionally, PGK1 may play a role in sperm motility, indicating potential contributions to cellular processes beyond its classic glycolytic function. The multifaceted roles attributed to PGK1 underscore its significance in cellular bioenergetics and suggest additional functions in cellular processes such as DNA replication and sperm motility.

Measured by NADH production in a reaction coupled with GAPDH. The specific activity is ≤115554.662 pmol/min/μg, as measured under the described conditions.

Human

HEK293

C-6*His

P00558-1 (S2-I417)

5230  [NCBI]

SLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

Approximately 46-50 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH 8.0 or 50 mM Tris, 300 mM NaCl, pH 8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.