PICALM Recombinant Protein,Human(HEK293,C-His)

PICALM Human Recombinant Protein (HEK293, C-His) plays a critical role in recruiting clathrin and adapter protein complex 2 (AP2) to the cell membrane during coated pit formation and clathrin-vesicle assembly effect. It regulates the size of the clathrin cage and determines membrane recycling. PICALM Recombinant Protein,Human (HEK293,C-His) is the recombinant human-derived PICALM Recombinant protein,Human(HEK293,C-His), expressed by HEK293 , with C-10*His labeled tag. The total length of PICALM Recombinant Protein,Human (HEK293,C-His) is 652 a.a., with molecular weight of ~90-150 kDa.

The PICALM gene encodes a clathrin assembly protein crucial for recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes during coated-pit formation and clathrin-vesicle assembly. Its role may extend to regulating the size of the clathrin cage, thus determining the amount of membrane to be recycled. This protein is implicated in AP2-dependent clathrin-mediated endocytosis, particularly at the neuromuscular junction. Notably, chromosomal translocation involving this gene is associated with various hematological malignancies. Additionally, polymorphisms in this gene are linked to Alzheimer's disease risk. The gene exhibits ubiquitous expression, with notable levels observed in fat, appendix, and 25 other tissues. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Human

HEK293

C-10*His

Q13492/NP_009097 (M1-M652)

8301

MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHPMSTASQVASTWGDPFSATVDAVDDAIPSLNPFLTKSSGDVHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNKSTNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLANLVGNLGIGNGTTKNDVNWSQPGEKKLTGGSNWQPKVAPTTAWNAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVSGAQIQFM

Approximately 90-150 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 10% glycerol, pH 7.3.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.