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  4. PKC iota Protein, Human (Sf9, His)

PKC iota Protein, Human (Sf9, His)

Cat. No.: HY-P702620
SDS COA Handling Instructions

PKC iota proteins are members of the calcium-dependent cell adhesion protein family and play critical roles in cellular interactions. PKC iota acts through a calcium-dependent mechanism and exhibits a preference for homogeneous interacting, connecting cells. PKC iota Protein, Human (Sf9, His) is the recombinant human-derived PKC iota, expressed by Sf9 insect cells , with C-His labeled tag. ,

For research use only. We do not sell to patients.

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5 μg $68 In-stock
10 μg $110 In-stock
50 μg $280 In-stock
100 μg $450 In-stock
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  • Biological Activity

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Description

PKC iota proteins are members of the calcium-dependent cell adhesion protein family and play critical roles in cellular interactions. PKC iota acts through a calcium-dependent mechanism and exhibits a preference for homogeneous interacting, connecting cells. PKC iota Protein, Human (Sf9, His) is the recombinant human-derived PKC iota, expressed by Sf9 insect cells , with C-His labeled tag. ,

Background

Cadherin-17, belonging to the family of calcium-dependent cell adhesion proteins, functions as a pivotal component in cellular interactions. Operating through a calcium-dependent mechanism, cadherins like CDH17 exhibit a propensity for homophilic interactions, preferentially engaging with themselves in connecting cells. This unique property positions cadherins to potentially contribute to the intricate sorting processes of heterogeneous cell types. Specifically, LI-cadherin, a subtype within this family, may play a crucial role in the morphological organization of vital organs such as the liver and intestine. This highlights the diverse and specialized functions that cadherins, including CDH17, fulfill in maintaining tissue integrity and structural organization within specific physiological contexts.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P41743 (M10-V596)

Gene ID

5584

Synonyms
Protein kinase C iota type; PRKC-lambda/iota; nPKC-iota; PRKCI; DXS1179E
Molecular Weight

90-110 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PKC iota Protein, Human (Sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PKC iota Protein, Human (Sf9, His)
Cat. No.:
HY-P702620
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