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  3. Transferases (EC 2)
  4. PRKCZ Protein, Human (Sf9, GST)

PRKCZ Protein, Human (Sf9, GST)

Cat. No.: HY-P701755
Handling Instructions

PKCz proteins are involved in multiple cellular processes, including mitotic signaling, cell proliferation, cell polarity, inflammatory responses, and maintenance of long-term potentiation (LTP). PRKCZ Protein, Human (Sf9, GST) is the recombinant human-derived PKCz protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of PRKCZ Protein, Human (Sf9, GST) is 591 a.a., .

For research use only. We do not sell to patients.

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Description

PKCz proteins are involved in multiple cellular processes, including mitotic signaling, cell proliferation, cell polarity, inflammatory responses, and maintenance of long-term potentiation (LTP). PRKCZ Protein, Human (Sf9, GST) is the recombinant human-derived PKCz protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of PRKCZ Protein, Human (Sf9, GST) is 591 a.a., .

Background

PKCz, a calcium- and diacylglycerol-independent serine/threonine-protein kinase, plays a multifaceted role in cellular processes, acting within the phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade. Involved in diverse functions such as NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response, and the maintenance of long-term potentiation (LTP), PKCz exhibits versatility in its cellular functions. In various contexts, it functions downstream of PI3K, independently activating the MAP2K1/MEK1-MAPK1/ERK2 signaling cascade, contributing to insulin-dependent activation of AKT3, and participating in the transactivation of NF-kappa-B. Additionally, PKCz plays a role in the establishment of cell polarity, stimulates neuronal differentiation, and is implicated in the development of allergic airway inflammation (asthma) through its involvement in Th2 immune response. Furthermore, PKCz is crucial in the late synaptic long-term potentiation phase in CA1 hippocampal cells and is associated with long-term memory maintenance.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

Q05513-1 (P2-V592)

Gene ID

5590

Molecular Construction
N-term
GST
PRKCZ (P2-V592)
Accession # Q05513-1
C-term
Synonyms
PRKCZ; Protein kinase C zeta type; nPKC-zeta
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PRKCZ Protein, Human (Sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PRKCZ Protein, Human (Sf9, GST)
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HY-P701755
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