PKM2 Protein, Mouse (Baculovirus, His)

PKM2 protein catalyzes the final step of glycolysis, transferring a phosphoryl group from PEP to ADP to generate ATP.In the nucleus, transcriptional activation of PKM2 contributes to cancer progression, promoting cell proliferation and tumorigenesis.PKM2 is also involved in caspase-independent cell death in tumors.PKM2 Protein, Mouse (Baculovirus, His) is the recombinant mouse-derived PKM2 protein, expressed by Sf9 insect cells , with N-10*His labeled tag.

The PKM2 protein assumes a multifaceted role in cellular processes, catalyzing the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The dynamic balance between its highly active tetrameric form and nearly inactive dimeric form dictates whether glucose carbons are directed towards biosynthetic processes or utilized for glycolytic ATP production, thereby contributing to the control of glycolysis. This transition between forms holds crucial significance for tumor cell proliferation and survival. An isoform expressed specifically during embryogenesis exhibits low pyruvate kinase activity by itself and necessitates allosteric activation by D-fructose 1,6-bisphosphate (FBP). Beyond its cytoplasmic pyruvate kinase activity, PKM2 functions as a transcriptional regulator in the nucleus, acting as a protein kinase. Upon translocation into the nucleus in response to various signals, such as EGF receptor activation, it homodimerizes, transforming into a protein threonine- and tyrosine-protein kinase. PKM2 also catalyzes the phosphorylation of STAT3 and histone H3, contributing to transcriptional activation. Its role in cancer cells involves promoting cell proliferation and tumorigenesis, along with regulating the expression of immune checkpoint proteins. Additionally, PKM2 acts as a translation regulator for specific mRNAs independently of its pyruvate kinase activity, associating with endoplasmic reticulum-associated ribosomes and promoting translation of endoplasmic reticulum-destined mRNAs. Furthermore, PKM2 plays a role in caspase-independent cell death in tumor cells. The diverse functionalities of PKM2 underscore its central position in coordinating crucial cellular processes with implications for metabolism, growth, and disease.

Measured by its ability to catalyze 1.25 mM ADP and 1.25 mM PEP that incubate at room temperature for 15 minutes. The specific activity is 16062.448 pmo/min/μg.

Mouse

Sf9 insect cells

N-10*His

P52480 (M1-P531)

18746  [NCBI]

MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Approximately 60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.