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Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)

Cat. No.: HY-P71613
SDS COA Handling Instructions

PLET1 protein orchestrates leading keratinocyte migration and enhances cellular adhesion to matrix proteins, crucial for wound healing. Its role extends to promoting efficient wound repair, emphasizing its significance in tissue regeneration. Additionally, PLET1 may influence trichilemmal differentiation in the hair follicle, indicating a broader impact on skin biology and homeostasis. Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Placenta-expressed transcript 1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is 191 a.a..

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  • Biological Activity

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Description

PLET1 protein orchestrates leading keratinocyte migration and enhances cellular adhesion to matrix proteins, crucial for wound healing. Its role extends to promoting efficient wound repair, emphasizing its significance in tissue regeneration. Additionally, PLET1 may influence trichilemmal differentiation in the hair follicle, indicating a broader impact on skin biology and homeostasis. Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is the recombinant mouse-derived Placenta-expressed transcript 1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) is 191 a.a..

Background

Placenta-expressed transcript 1 (PLET1) protein plays a pivotal role in orchestrating leading keratinocyte migration and facilitating cellular adhesion to matrix proteins, thereby contributing to the intricate process of wound healing. Its involvement extends to promoting efficient wound repair, underscoring its significance in tissue regeneration. Furthermore, PLET1 may exert its influence during trichilemmal differentiation within the context of the hair follicle, suggesting a broader impact on skin biology and homeostasis.

Biological Activity

Immobilized Mouse Placenta-expressed transcript 1 at 1 μg/mL (100 μL/well) can bind Anti- Placenta-expressed transcript 1. The ED50 for this effect is 0.03 μg/mL.

Species

Mouse

Source

E. coli

Tag

N-His;N-SUMO

Accession

Q8VEN2-1 (S28-S218)

Gene ID
Molecular Construction
N-term
6*His-SUMO
PLET1 (S28-S218)
Accession # Q8VEN2
C-term
Synonyms
Plet1; Placenta-expressed transcript 1 protein; Antigen mAgK114
AA Sequence

SDNGSCVVLDNIYTSDILEISTMANVSGGDVTYTVTVPVNDSVSAVILKAVKEDDSPVGTWSGTYEKCNDSSVYYNLTSQSQSVFQTNWTVPTSEDVTKVNLQVLIVVNRTASKSSVKMEQVQPSASTPIPESSETSQTINTTPTVNTAKTTAKDTANTTAVTTANTTANTTAVTTAKTTAKSLAIRTLGS

Molecular Weight

Approximately 43 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 6% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Placenta-expressed transcript 1 Protein, Mouse (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Placenta-expressed transcript 1 Protein, Mouse (His-SUMO)
Cat. No.:
HY-P71613
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