PLAU/uPA Protein, Mouse (HEK293, C-His-Avi)

PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-His-Avi labeled tag.

PLAU, also known as urokinase-type plasminogen activator (uPA), plays a crucial role in fibrinolysis by specifically cleaving the zymogen plasminogen to generate the active enzyme plasmin. This enzymatic conversion is a pivotal step in the regulation of blood clot dissolution and tissue remodeling. PLAU's ability to activate plasmin sets in motion a cascade of proteolytic events, contributing to the breakdown of fibrin clots and extracellular matrix components. This process is essential for various physiological functions, including wound healing, tissue repair, and the resolution of blood clots.

Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Mouse PLAU, His-Avi Tag with an affinity constant of 0.33-10.24 μM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-Avi;C-His

P06869 (G21-F433)

18792  [NCBI]

GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF

30-35 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 or 20mM PB, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.