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  4. Pleckstrin Protein, Human (His)

Pleckstrin Protein, Human (His)

Cat. No.: HY-P73707
Handling Instructions

Pleckstrin Protein serves as a primary substrate for protein kinase C in platelets. Pleckstrin Protein, Human (His) is the recombinant human-derived Pleckstrin protein, expressed by E. coli , with N-His labeled tag. The total length of Pleckstrin Protein, Human (His) is 350 a.a., with molecular weight of ~45 kDa.

For research use only. We do not sell to patients.

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Description

Pleckstrin Protein serves as a primary substrate for protein kinase C in platelets. Pleckstrin Protein, Human (His) is the recombinant human-derived Pleckstrin protein, expressed by E. coli , with N-His labeled tag. The total length of Pleckstrin Protein, Human (His) is 350 a.a., with molecular weight of ~45 kDa.
Background

Pleckstrin stands out as a major substrate for protein kinase C within platelets.
Species

Human
Source

E. coli
Tag

N-His
Accession

P08567 (M1-K350)
Gene ID
Molecular Construction
N-term
His
Pleckstrin (M1-K350)
Accession # P08567
C-term
Synonyms
Pleckstrin; p47; PLEK; Platelet 47 kDa protein
Molecular Weight

Approximately 45 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Pleckstrin Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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