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PNLIPRP1 Protein, Rat (HEK293, His)

Cat. No.: HY-P77464
SDS COA Handling Instructions

PNLIPRP1 Protein, a potential inhibitor of dietary triglyceride digestion, likely modulates lipid metabolism. In vitro analyses reveal its lack of detectable lipase activity, suggesting a unique role as an enzyme inhibitor rather than an active participant in triglyceride hydrolysis. This characteristic underscores PNLIPRP1's potential regulatory impact on lipid digestion processes. PNLIPRP1 Protein, Rat (HEK293, His) is the recombinant rat-derived PNLIPRP1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

PNLIPRP1 Protein, a potential inhibitor of dietary triglyceride digestion, likely modulates lipid metabolism. In vitro analyses reveal its lack of detectable lipase activity, suggesting a unique role as an enzyme inhibitor rather than an active participant in triglyceride hydrolysis. This characteristic underscores PNLIPRP1's potential regulatory impact on lipid digestion processes. PNLIPRP1 Protein, Rat (HEK293, His) is the recombinant rat-derived PNLIPRP1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The PNLIPRP1 Protein emerges as a potential inhibitor of dietary triglyceride digestion, showcasing its probable role in modulating lipid metabolism. Notably, it lacks detectable lipase activity in vitro, as suggested by similarity analyses. This characteristic hints at PNLIPRP1's unique function as an enzyme inhibitor rather than an active participant in triglyceride hydrolysis, providing valuable insights into its potential regulatory role in lipid digestion processes.

Species

Rat

Source

HEK293

Tag

C-His

Accession

P54316 (K18-V473)

Gene ID
Molecular Construction
N-term
PNLIPRP1 (K18-V473)
Accession # P54316
His
C-term
Synonyms
PNLIPRP1; PLRP1; Inactive pancreatic lipase-related protein 1; PL-RP1
AA Sequence

KEVCYDNLGCFSDAEPWAGTAIRPLKLLPWSPEKINTRFLLYTNENPTAFQTLQLSDPLTIGASNFQVARKTRFIIHGFIDKGEENWVVDMCKNMFQVEEVNCICVDWKKGSQTTYTQAANNVRVVGAQVAQMIDILVKNYSYSPSKVHLIGHSLGAHVAGEAGSRTPGLGRITGLDPVEANFEGTPEEVRLDPSDADFVDVIHTDAAPLIPFLGFGTNQMSGHLDFFPNGGQSMPGCKKNALSQIVDIDGIWSGTRDFVACNHLRSYKYYLESILNPDGFAAYPCASYKDFESNKCFPCPDQGCPQMGHYADKFAGKSGDEPQKFFLNTGEAKNFARWRYRVSLILSGRMVTGQVKVALFGSKGNTRQYDIFRGIIKPGATHSSEFDAKLDVGTIEKVKFLWNNQVINPSFPKVGAAKITVQKGEERTEYNFCSEETVREDTLLTLLPCETSDTV

Molecular Weight

Approximately 55-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PNLIPRP1 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PNLIPRP1 Protein, Rat (HEK293, His)
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HY-P77464
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