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  3. PNO1 Protein, Human (His)

PNO1 Protein, Human (His)

Cat. No.: HY-P702824
COA Handling Instructions

PNO1 Protein, Human (His) is the recombinant human-derived PNO1, expressed by E. coli , with C-6*His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $180 In-stock
50 μg $345 In-stock
100 μg $550 In-stock
> 100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PNO1 Protein, Human (His) is the recombinant human-derived PNO1, expressed by E. coli , with C-6*His labeled tag. ,

Biological Activity

Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is typically 0.4094 μg/mL, corresponding to a specific activity is 2442.599 units/mg.

  • Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is typically 0.4094 μg/mL, corresponding to a specific activity is 2442.599 units/mg.
Species

Human

Source

E. coli

Tag

C-6*His

Accession

Q9NRX1 (M1-F252)

Gene ID

56902

Synonyms
RNA-binding protein PNO1; Partner of NOB1
AA Sequence

MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF

Molecular Weight

Approximately 35 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 10% trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please reconstitute on ice in PBS, 10% trehalose, pH 8.0 buffer.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PNO1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PNO1 Protein, Human (His)
Cat. No.:
HY-P702824
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