POLD2 Protein, Human (His)

The POLD2 protein is a key component of DNA polymerase delta (Pol-delta) and DNA polymerase zeta (Pol-zeta) complexes, which are essential for genome replication fidelity and repair. In Pol-delta3 and Pol-delta4 forms, POLD2 contributes to high-fidelity lagging strand synthesis. POLD2 Protein, Human (His) is the recombinant human-derived POLD2, expressed by E. coli , with C-6*His labeled tag. ,

POLD2, an integral component of both the DNA polymerase delta (Pol-delta) complex and the DNA polymerase zeta (Pol-zeta) complex, plays a pivotal role in genome replication fidelity and repair processes. Within the trimeric and tetrameric forms of Pol-delta (Pol-delta3 and Pol-delta4), POLD2 contributes to high-fidelity lagging strand synthesis and participates in the replication and repair of the genome. Notably, the catalytic activities and substrate preferences of Pol-delta3 and Pol-delta4 vary, with Pol-delta3 exhibiting superior proofreading capabilities. POLD2 is crucial for nucleotide excision repair (NER) synthesis following UV irradiation and is involved in translesion synthesis (TLS) of damaged templates. Additionally, POLD2, along with POLD3, enhances the efficiency and processivity of DNA synthesis in the Pol-zeta complex, impacting various cellular functions related to DNA replication and repair. Furthermore, POLD2 interacts with a range of proteins, such as WRNIP1, KCTD13/PDIP1, and KCTD10, underscoring its regulatory role in DNA polymerase activities and cellular responses to DNA damage.

Human

E. coli

C-6*His

P49005 (M1-P469)

5425  [NCBI]

MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Approximately 53 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 0.1% SKL.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.