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  3. POLD2 Protein, Human (His)

The POLD2 protein is a key component of DNA polymerase delta (Pol-delta) and DNA polymerase zeta (Pol-zeta) complexes, which are essential for genome replication fidelity and repair. In Pol-delta3 and Pol-delta4 forms, POLD2 contributes to high-fidelity lagging strand synthesis. POLD2 Protein, Human (His) is the recombinant human-derived POLD2, expressed by E. coli , with C-6*His labeled tag. ,

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Description

The POLD2 protein is a key component of DNA polymerase delta (Pol-delta) and DNA polymerase zeta (Pol-zeta) complexes, which are essential for genome replication fidelity and repair. In Pol-delta3 and Pol-delta4 forms, POLD2 contributes to high-fidelity lagging strand synthesis. POLD2 Protein, Human (His) is the recombinant human-derived POLD2, expressed by E. coli , with C-6*His labeled tag. ,

Background

POLD2, an integral component of both the DNA polymerase delta (Pol-delta) complex and the DNA polymerase zeta (Pol-zeta) complex, plays a pivotal role in genome replication fidelity and repair processes. Within the trimeric and tetrameric forms of Pol-delta (Pol-delta3 and Pol-delta4), POLD2 contributes to high-fidelity lagging strand synthesis and participates in the replication and repair of the genome. Notably, the catalytic activities and substrate preferences of Pol-delta3 and Pol-delta4 vary, with Pol-delta3 exhibiting superior proofreading capabilities. POLD2 is crucial for nucleotide excision repair (NER) synthesis following UV irradiation and is involved in translesion synthesis (TLS) of damaged templates. Additionally, POLD2, along with POLD3, enhances the efficiency and processivity of DNA synthesis in the Pol-zeta complex, impacting various cellular functions related to DNA replication and repair. Furthermore, POLD2 interacts with a range of proteins, such as WRNIP1, KCTD13/PDIP1, and KCTD10, underscoring its regulatory role in DNA polymerase activities and cellular responses to DNA damage.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P49005 (M1-P469)

Gene ID
Molecular Construction
N-term
POLD2 (M1-P469)
Accession # P49005
6*His
C-term
Synonyms
DNA Polymerase Delta Subunit 2; DNA Polymerase Delta Subunit p50; POLD2
AA Sequence

MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP

Molecular Weight

Approximately 53 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

POLD2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
POLD2 Protein, Human (His)
Cat. No.:
HY-P71220
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