1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PPA1 Protein, S. cerevisiae (His)

PPA1 Protein, S. cerevisiae (His)

Cat. No.: HY-P79310
SDS COA Handling Instructions

The PPA1 protein is an important member of the PPase family and plays an important role in cellular processes, especially dephosphorylation related to phosphate metabolism. PPA1 shares conserved features with related proteins and is involved in phosphatase activity. PPA1 Protein, S. cerevisiae (His) is the recombinant human-derived PPA1 protein, expressed by E. coli , with tag free. The total length of PPA1 Protein, S. cerevisiae (His) is 287 a.a., with molecular weight of ~34.19 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The PPA1 protein is an important member of the PPase family and plays an important role in cellular processes, especially dephosphorylation related to phosphate metabolism. PPA1 shares conserved features with related proteins and is involved in phosphatase activity. PPA1 Protein, S. cerevisiae (His) is the recombinant human-derived PPA1 protein, expressed by E. coli , with tag free. The total length of PPA1 Protein, S. cerevisiae (His) is 287 a.a., with molecular weight of ~34.19 kDa.

Background

The PPA1 Protein is an integral member of the PPase family, highlighting its significant role in cellular processes. As part of this enzyme family, PPA1 likely shares conserved structural and functional features with related proteins, signifying its involvement in phosphatase activities. The classification within the PPase family underscores its specific designation within the broader context of phosphatases, providing insights into its unique enzymatic functions. The study of PPA1 contributes to our understanding of its role in cellular processes related to dephosphorylation, offering potential applications in therapeutic interventions and a deeper comprehension of its broader impact on cellular processes involved in phosphate metabolism. Further exploration of PPA1's role holds promise for enhancing our knowledge of its contributions to both normal physiology and pathological conditions.

Biological Activity

Measured by its ability to hydrolyze pyrophosphate. The specific activity is 167023 pmol/min/μg, as measured under the described conditions.

Species

Others

Source

E. coli

Tag

Tag Free

Accession

A6ZKV8 (M1-V287)

Gene ID

852296  [NCBI]

Molecular Construction
N-term
PPA1 (M1-V287)
Accession # A6ZKV8
C-term
Synonyms
Inorganic pyrophosphatase; IPP1; Pyrophosphate phospho-hydrolase; PPase; PPA; PPA1
AA Sequence

MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV

Molecular Weight

Approximately 34.19 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE
Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 10 mM Tris, 50 mM NaCl, 1 mM DDT, 0.1 mM EDTA, 50% Glycerol, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PPA1 Protein, S. cerevisiae (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PPA1 Protein, S. cerevisiae (His)
Cat. No.:
HY-P79310
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