PPC-DC Protein, Human (His, solution)

PPC-DC proteins are central players in cellular ubiquitination, covalently linking ubiquitin to target proteins. Its critical role extends to the selective degradation of misfolded membrane proteins, an important process in endoplasmic reticulum-associated degradation (ERAD). PPC-DC Protein, Human (His, solution) is the recombinant human-derived PPC-DC protein, expressed by E. coli , with N-6*His labeled tag.

UBE2J2 protein serves as a key player in cellular ubiquitination processes, facilitating the covalent attachment of ubiquitin to target proteins. Its functional significance extends to the selective degradation of misfolded membrane proteins, a crucial aspect of endoplasmic reticulum-associated degradation (ERAD). This involvement underscores UBE2J2's role in maintaining cellular homeostasis by actively participating in the quality control of protein folding within the endoplasmic reticulum. Additionally, in collaboration with the GATOR2 complex, UBE2J2 catalyzes the 'Lys-6'-linked ubiquitination of NPRL2, further emphasizing its regulatory influence on specific cellular pathways, particularly those related to protein quality control.

Human

E. coli

N-6*His

Q96CD2-1 (M1-S204)

60490  [NCBI]

MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.