PPID Protein, Human (His)

PPID protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and contributes to protein folding. It serves as a co-chaperone in the HSP90 complex, contributing to dynamic regulation and may favor the estrogen receptor complex. PPID Protein, Human (His) is the recombinant human-derived PPID protein, expressed by E. coli , with N-6*His, C-6*His labeled tag.

PPID Protein serves as a peptidyl-prolyl cis-trans isomerase (PPIase), catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and potentially aiding in protein folding processes. Proposed to act as a co-chaperone in HSP90 complexes, particularly in unligated steroid receptors heterocomplexes, PPID contributes to the dynamic regulation of HSP90-co-chaperone-receptor complexes. This involvement suggests a role in tissue-specific control of receptor activity, with a potential preference for estrogen receptor complexes over glucocorticoid receptor complexes. Moreover, PPID may participate in the cytoplasmic dynein-dependent movement of receptors from the cytoplasm to the nucleus. Notably, it is implicated in regulating MYB by inhibiting its DNA-binding activity and plays a role in AHR signaling by promoting the formation of the AHR:ARNT dimer, independently of HSP90 but requiring chaperone activity. Additionally, PPID is involved in the regulation of UV radiation-induced apoptosis and promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines. In the context of microbial infection, PPID may be associated with hepatitis C virus (HCV) replication and release.

Measured by its ability to convert 3mM substrate, Suc-AAPF-pNA (HY-P2573), from Cis to Trans formation. That incubate at 37℃ temperature in kinetic mode for 1minutes. The specific activity is 1536.201 pmol/min/μg.

Human

E. coli

C-6*His;N-6*His

Q08752 (M1-A370)

5481

MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

Approximately 43 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.