PRKAR1A Protein, Human (HEK293, His)

PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase and is critical for cAMP signaling. Upon activation by cAMP, it releases two catalytic monomers and a regulatory dimer from the inactive holoenzyme. PRKAR1A Protein, Human (HEK293, His) is the recombinant human-derived PRKAR1A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRKAR1A Protein, Human (HEK293, His) is 380 a.a., with molecular weight of ~50.0 kDa.

PRKAR1A Protein, a regulatory subunit of cAMP-dependent protein kinases, plays a crucial role in cAMP signaling within cells. The inactive holoenzyme consists of two regulatory chains and two catalytic chains, which are released upon activation by cAMP, resulting in the formation of two active catalytic monomers and a regulatory dimer. PRKAR1A Protein interacts with PRKACA and PRKACB, and this interaction has been shown to influence various cellular processes. Additionally, PRKAR1A Protein engages with RFC2, potentially contributing to cell survival. It also interacts with AKAP4, RARA (in the presence of cAMP or FSH, regulating RARA transcriptional activity), phosphorylated PJA2, CBFA2T3 (By similarity), PRKX (regulating this cAMP-dependent protein kinase), C2orf88/smAKAP (possibly targeting PRKAR1A to the plasma membrane), and AICDA, further highlighting its involvement in diverse molecular interactions and pathways.

Human

HEK293

C-6*His

P10644 (E2-V381)

5573  [NCBI]

ESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.