1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Betacellulin
  5. Betacellulin Protein, Human

Betacellulin Protein, Human

Cat. No.: HY-P7005
COA Handling Instructions

Probetacellulin Protein, Human is a glycoprotein, improves glucose tolerance by promoting β-cell differentiation and regeneration from ductal and/or acinar cells.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $140 In-stock
50 μg $420 In-stock
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

Probetacellulin Protein, Human is a glycoprotein, improves glucose tolerance by promoting β-cell differentiation and regeneration from ductal and/or acinar cells.

Background

Betacellulin (BTC), a member of the epidermal growth factor family, is expressed predominantly in the human pancreas and induces the differentiation of a pancreatic acinar cell line (AR42J) into insulin-secreting cells. Betacellulin binds and activates the EGF receptor (EGFR/erbB-1) and erbB-4, and it induces tyrosine phosphorylation of erbB-2, which couples with the EGFR or erbB-4[1].

Biological Activity

The ED50 is <0.01 ng/mL as measured by murine Balb/3T3 cells, corresponding to a specific activity of >1.0 × 108 units/mg.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P35070 (D32-Y111)

Gene ID

685  [NCBI]

Molecular Construction
N-term
Betacellulin (D32-Y111)
Accession # P35070
C-term
Synonyms
rHuBetacellulin; Probetacellulin; BTC
AA Sequence

DGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFY

Molecular Weight

Approximately 15 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Betacellulin Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Betacellulin Protein, Human
Cat. No.:
HY-P7005
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