Profilin-4 Protein, Human (His)

The profilin-4 protein is critical for male fertility and plays an important role in manchette development and acrosome biogenesis during spermatogenesis. It has binding affinity for a variety of phospholipids, including PtdIns(3)P, PtdIns(4,5)P2, PtdIns(4)P, and PA. Profilin-4 Protein, Human (His) is the recombinant human-derived Profilin-4 protein, expressed by E. coli , with N-His labeled tag. The total length of Profilin-4 Protein, Human (His) is 129 a.a., with molecular weight of ~14 kDa.

Profilin-4 protein plays a crucial role in male fertility, being essential for manchette development and acrosome biogenesis during spermiogenesis. This protein exhibits binding affinity in vitro to various phospholipids, including phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidic acid (PA). Notably, unlike other members of the profilin family, Profilin-4 does not bind to actin in vitro. These distinctive properties underscore its specialized functions in the intricate processes of male reproductive physiology, particularly in the development of manchette and acrosome during sperm maturation.

Human

E. coli

N-6*His

Q8NHR9 (M1-S129)

375189  [NCBI]

MSHLQSLLLDTLLGTKHVDSAALIKIQERSLCVASPGFNVTPSDVRTLVNGFAKNPLQARREGLYFKGKDYRCVRADEYSLYAKNENTGVVVVKTHLYLLVATYTEGMYPSICVEATESLGDYLRKKGS

Approximately 14 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.