Progranulin/PGRN Protein, Mouse (HEK293, His)

Progranulin (PGRN) proteins regulate lysosomal function, affecting protein transport, enzymatic activity, and acidification. It degrades mature cathepsin D via cathepsin B. Progranulin/PGRN Protein, Mouse (572a.a, HEK293, His) is the recombinant mouse-derived Progranulin/PGRN protein, expressed by HEK293 , with C-His labeled tag. The total length of Progranulin/PGRN Protein, Mouse (572a.a, HEK293, His) is 572 a.a., with molecular weight of 70-90 kDa.

Progranulin (PGRN) Protein operates as a crucial regulator of lysosomal function and a growth factor with roles spanning inflammation, wound healing, and cell proliferation. PGRN orchestrates protein trafficking to lysosomes, influences lysosomal enzyme activity, and promotes lysosomal acidification, culminating in the degradation of mature cathepsin D by cathepsin B. Beyond its lysosomal functions, PGRN acts as a growth factor in wound healing, directly impacting dermal fibroblasts and endothelial cells, fostering cell division, migration, and the formation of capillary-like tubule structures. It further contributes to epithelial cell proliferation by impeding TNF-mediated neutrophil activation, preventing the release of oxidants and proteases. Additionally, PGRN plays a pivotal role in modulating inflammation in neurons, safeguarding neuronal survival, axonal outgrowth, and overall neuronal integrity. However, it exhibits a contrasting effect on epithelial cells, inhibiting their proliferation and prompting the secretion of IL-8. The multifaceted actions of PGRN highlight its versatile functions in cellular processes with implications for various physiological and pathological conditions.

Measured by its ability to inhibit THP-1 human acute monocyte migration in the presence of 10 ng/mL MCP-1.

Mouse

HEK293

C-His

P28798 (T18-L589)

14824  [NCBI]

TQCPDGQFCPVACCLDQGGANYSCCNPLLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDPVPRPLL

70-90 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.