Prolactin Protein, Human (HEK293, His)

Prolactin Protein acts on the mammary gland, promoting lactation through its interaction with the receptor PRLR. Prolactin Protein, Human (HEK293, His) is the recombinant human-derived Prolactin protein, expressed by HEK293 , with C-His labeled tag. The total length of Prolactin Protein, Human (HEK293, His) is 199 a.a., with molecular weight of 25-30 kDa.

Prolactin is a protein that primarily acts on the mammary gland by promoting lactation. It achieves this by interacting with its receptor, PRLR.

Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED₅₀ this effect is 0.1656-0.4161 ng/mL, corresponding to a specific activity is 6.038×10⁶ units/mg.

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  Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED₅₀ for this effect is 0.1656 ng/mL, corresponding to a specific activity is 6.038×10⁶ units/mg.

Human

HEK293

C-His

P01236 (L29-C227)

5617  [NCBI]

LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC

Approximately 24-30 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.