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  2. Receptor Proteins
  3. Receptor Serine/Threonine Kinases
  4. Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST)

Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST)

Cat. No.: HY-P74615
Handling Instructions

Protein kinase D2 (PRKD2) is a serine/threonine protein kinase that mediates various cellular processes downstream of PKC. It affects cell proliferation through MAPK1/3 (ERK1/2) signaling, activates NF-kappa-B under oxidative stress, inhibits HDAC7 transcriptional repression, and regulates T cell antigen receptor (TCR) signaling, cytokine production, Golgi trafficking, angiogenesis, particle release, and cell adhesion. Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST) is the recombinant human-derived protein Kinase D2/PRKD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.

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Description

Protein kinase D2 (PRKD2) is a serine/threonine protein kinase that mediates various cellular processes downstream of PKC. It affects cell proliferation through MAPK1/3 (ERK1/2) signaling, activates NF-kappa-B under oxidative stress, inhibits HDAC7 transcriptional repression, and regulates T cell antigen receptor (TCR) signaling, cytokine production, Golgi trafficking, angiogenesis, particle release, and cell adhesion. Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST) is the recombinant human-derived protein Kinase D2/PRKD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

Protein Kinase D2 (PRKD2), a serine/threonine-protein kinase, acts as a pivotal mediator of diverse cellular processes. It translates transient diacylglycerol (DAG) signals into sustained physiological effects downstream of PKC, influencing cell proliferation through MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, T-cell antigen receptor (TCR) and cytokine production, Golgi membrane trafficking, angiogenesis, secretory granule release, and cell adhesion. PRKD2 may enhance mitogenesis induced by neuropeptide bombesin by prolonging MAPK1/3 signaling, leading to the accumulation of immediate-early gene products, including FOS, promoting cell cycle progression. Phosphorylation of PRKD2 at Tyr-438 and Tyr-717 by ABL1 in response to oxidative stress activates NF-kappa-B. Additionally, PRKD2, in response to gastrin receptor CCKBR activation, phosphorylates HDAC7, inhibiting its transcriptional repression of NR4A1/NUR77. The kinase also plays essential roles in T-cell activation, angiogenesis, secretion, and tumor growth, demonstrating its multifaceted involvement in cellular functions.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q9BZL6-1/ NP_057541.2 (M1-L878)

Gene ID
Molecular Construction
N-term
His-GST
NP_057541.2 Protein Kinase D2/PRKD2 (M1-L878)
Accession # Q9BZL6-1/
C-term
Synonyms
Serine/threonine-protein kinase D2; PRKD2; PKD2; HSPC187
Molecular Weight

Approximately 120 kDa

Purity

Greater than 82% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Protein Kinase D2/PRKD2 Protein, Human (sf9, His-GST)
Cat. No.:
HY-P74615
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