Prothrombin Protein, Human (HEK293, His)

Prothrombin or factor II is a key protein in a variety of physiological processes. The serine protease thrombin, derived from prothrombin, cleaves the bond behind arginine and lysine residues, converting fibrinogen to fibrin. Prothrombin Protein, Human (HEK293, His) is the recombinant human-derived Prothrombin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Prothrombin Protein, Human (HEK293, His) is 622 a.a., with molecular weight of ~75-100 kDa.

The Prothrombin protein, also known as Factor II, serves as a critical player in various physiological processes. Thrombin, a serine protease derived from prothrombin, cleaves bonds following Arg and Lys residues, facilitating the conversion of fibrinogen into fibrin. Additionally, prothrombin actively participates in the activation of key factors such as V, VII, VIII, XIII, and, when in conjunction with thrombomodulin, protein C. Beyond its central role in blood homeostasis, prothrombin contributes significantly to inflammation and the intricate process of wound healing.

Human

HEK293

C-10*His

P00734 (M1-E622)

2147

MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE

Approximately 75-100 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from sterile 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.