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Prothymosin alpha Protein, Rat (P.pastoris, His)

Cat. No.: HY-P71776
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Thymosin alpha protein is a multifunctional player that may mediate immune function by fighting opportunistic infections. It binds to NUPR1, suggesting regulation of the apoptotic process. Prothymosin alpha Protein, Rat (P.pastoris, His) is the recombinant rat-derived Prothymosin alpha protein, expressed by P. pastoris , with N-His labeled tag.

For research use only. We do not sell to patients.

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Description

Thymosin alpha protein is a multifunctional player that may mediate immune function by fighting opportunistic infections. It binds to NUPR1, suggesting regulation of the apoptotic process. Prothymosin alpha Protein, Rat (P.pastoris, His) is the recombinant rat-derived Prothymosin alpha protein, expressed by P. pastoris , with N-His labeled tag.

Background

Prothymosin alpha, a multifunctional protein, potentially plays a pivotal role in mediating immune function by conferring resistance against specific opportunistic infections. Through its intricate molecular interactions, prothymosin alpha engages with NUPR1, suggesting its involvement in the regulation of apoptotic processes. The multifaceted functions of prothymosin alpha underscore its significance in orchestrating immune responses and cellular homeostasis. Further elucidation of its molecular interactions and regulatory roles can provide valuable insights into the complex mechanisms through which prothymosin alpha contributes to immune function and apoptotic regulation in cellular contexts.

Species

Rat

Source

P. pastoris

Tag

N-6*His

Accession

P06302 (S2-D112)

Gene ID

100359583  [NCBI]

Molecular Construction
N-term
His
ProTα (S2-D112)
Accession # P06302
C-term
Synonyms
Ptma; N-terminally processed
AA Sequence

SDAAVDTSSEITTKDLKEKKEVVEEAENGRDAPANGNAQNEENGEQEADNEVDEEEEEGGEEEEEEEEGDGEEEDGDEDEEAEAPTGKRVAEDDEDDDVETKKQKKTDEDD

Molecular Weight

Approximately 14.3 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Prothymosin alpha Protein, Rat (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Prothymosin alpha Protein, Rat (P.pastoris, His)
Cat. No.:
HY-P71776
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