PTGDS Protein, Human (HEK293, His)

The PTGDS protein catalyzes the conversion of PGH2 to PGD2, a prostaglandin that affects smooth muscle activity and platelet aggregation. It plays a role in central nervous system functions such as sedation, sleep, and allodynia. PTGDS Protein, Human (HEK293, His) is the recombinant human-derived PTGDS protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PTGDS Protein, Human (HEK293, His) is 168 a.a., with molecular weight of 20-29 kDa.

The PTGDS protein serves a multifaceted role in various physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin with implications in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its involvement in these vascular functions, PTGDS plays a crucial role in diverse central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exhibit an anti-apoptotic function in oligodendrocytes. Notably, PTGDS has a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules such as biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone. This suggests its potential role as a scavenger for harmful hydrophobic molecules and as a transporter for secretory retinoids and thyroid hormones. Additionally, PTGDS is implicated in the development and maintenance of various blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, indicating its significance in barrier function. Furthermore, it plays essential roles in the maturation and maintenance of the central nervous system and the male reproductive system, as well as participates in PLA2G3-dependent maturation of mast cells, further emphasizing its intricate involvement in diverse biological processes.

Measured by its ability to catalyse the conversion of PGH2 to PGD2. The specific activity is 5.207 pg/min/µg that incubate at 25 ºC for 1 minutes.

Human

HEK293

C-6*His

P41222 (A23-Q190)

5730  [NCBI]

APEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQTDKCMTEQ

20-29 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10%Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.