PTGDS Protein, Human (HEK293, C-His)

The PTGDS protein serves a multifaceted role in various physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin with implications in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its involvement in these vascular functions, PTGDS plays a crucial role in diverse central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exhibit an anti-apoptotic function in oligodendrocytes. Notably, PTGDS has a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules such as biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone. This suggests its potential role as a scavenger for harmful hydrophobic molecules and as a transporter for secretory retinoids and thyroid hormones. Additionally, PTGDS is implicated in the development and maintenance of various blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, indicating its significance in barrier function. Furthermore, it plays essential roles in the maturation and maintenance of the central nervous system and the male reproductive system, as well as participates in PLA2G3-dependent maturation of mast cells, further emphasizing its intricate involvement in diverse biological processes. PTGDS Protein, Human (HEK293, C-His) is the recombinant human-derived PTGDS protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PTGDS Protein, Human (HEK293, C-His) is 168 a.a., with molecular weight of ~21 kDa.

The PTGDS protein serves a multifaceted role in various physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin with implications in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its involvement in these vascular functions, PTGDS plays a crucial role in diverse central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exhibit an anti-apoptotic function in oligodendrocytes. Notably, PTGDS has a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules such as biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone. This suggests its potential role as a scavenger for harmful hydrophobic molecules and as a transporter for secretory retinoids and thyroid hormones. Additionally, PTGDS is implicated in the development and maintenance of various blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, indicating its significance in barrier function. Furthermore, it plays essential roles in the maturation and maintenance of the central nervous system and the male reproductive system, as well as participates in PLA2G3-dependent maturation of mast cells, further emphasizing its intricate involvement in diverse biological processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-10*His

P41222 (A23-Q190)

5730  [NCBI]

APEAQVSVQPNFQQDKFLGRWFSAGLASNSSWLREKKAALSMCKSVVAPATDGGLNLTSTFLRKNQCETRTMLLQPAGSLGSYSYRSPHWGSTYSVSVVETDYDQYALLYSQGSKGPGEDFRMATLYSRTQTPRAELKEKFTAFCKAQGFTEDTIVFLPQTDKCMTEQ

Approximately 21 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.