PTHrP Protein, Human (His)

The PTHrP protein is a key neuroendocrine peptide that regulates multiple cellular processes, including growth, development, migration, differentiation, and survival. It plays an integral role in epithelial calcium transport and key developmental events such as endochondral bone development, mammary gland, and tooth formation. PTHrP Protein, Human (His) is the recombinant human-derived PTHrP protein, expressed by E. coli , with N-6*His labeled tag. The total length of PTHrP Protein, Human (His) is 139 a.a., with molecular weight of 19.7 kDa.

Parathyroid hormone-related protein (PTHrP) emerges as a pivotal neuroendocrine peptide that orchestrates diverse cellular processes, encompassing growth, development, migration, differentiation, and survival, along with its integral role in epithelial calcium ion transport. Its regulatory influence extends to critical developmental events, such as endochondral bone development and the intricate interactions shaping mammary gland and tooth formation. PTHrP's indispensable contribution to skeletal homeostasis underscores its significance in maintaining bone integrity. Within the mammary context, PTHrP plays a multifaceted role, driving mesenchymal differentiation, promoting bud outgrowth, and modulating responsiveness to bone morphogenetic proteins (BMPs), thus intricately shaping mammary tissue architecture. Additionally, PTHrP influences colon cancer cell behavior, enhancing migration and invasion through integrin alpha-6/beta-1-dependent mechanisms. Notably, osteostatin, a derivative of PTHrP, exhibits potent inhibitory effects on osteoclastic bone resorption, emphasizing its role in bone metabolism regulation.

Measured by its binding ability in a functional ELISA. Immobilized Human PTH1R at 2μg/mL (100μl/well) on the plate can bind Biotinylated Human PTHrP. The ED₅₀ for this effect is 0.0979 μg/mL.

Human

E. coli

N-6*His

P12272 (A37-R175)

5744

19.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of Tris-based buffer,50% glycerol or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.