PTPRC/CD45R0 Protein, Human (HEK293, His)

PTPRC/CD45R0 Protein, Human (HEK 293, His) expresses in HEK293 with a His tag at the N-terminus. CD45R0 (UCHL1), a member of leucocyte common antigen family, is expressed largely on previously activated or memory T cells^[1][2].

CD45 regulates the interaction between T cells and macrophages via its ligand macrophage galactose-type lectin which binds to CD45 N-acetylgalactosamine and results in decreasing the level of T cell proliferation with proinflammatory cytokine production leading to T cell apoptosis.

Human

HEK293

C-6*His

P08575-4 (Q26-K577)

5788  [NCBI]

QSPTPSPTDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK

80-120 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Maryam Ahmed Al Barashdi, et al. Protein tyrosine phosphatase receptor type C (PTPRC or CD45). J Clin Pathol. 2021 Sep;74(9):548-552.  [Content Brief]

  [2]. T Miyawaki, et al. Expression of CD45R0 (UCHL1) by CD4+ and CD8+ T cells as a sign of in vivo activation in infectious mononucleosis. Clin Exp Immunol. 1991 Mar;83(3):447-51.  [Content Brief]