PXR Protein, Human (His)

The PXR protein is a nuclear receptor that acts as a multifunctional transcription factor activated by a variety of endogenous and exogenous compounds. It regulates genes involved in the metabolism and secretion of substances, responding to ligands such as rifampicin, hypericin, gugulin, colupulone, isoflavones, pregnenolone and progesterone. PXR Protein, Human (His) is the recombinant human-derived PXR protein, expressed by E. coli , with N-6*His labeled tag. The total length of PXR Protein, Human (His) is 305 a.a., .

PXR Protein, a nuclear receptor, serves as a versatile transcription factor activated by a range of endogenous and xenobiotic compounds. It plays a pivotal role in regulating the transcription of multiple genes involved in the metabolism and secretion of potentially harmful substances, including xenobiotics, drugs, and endogenous compounds. Activated by various ligands such as the antibiotic rifampicin, as well as plant metabolites like hyperforin, guggulipid, colupulone, and isoflavones, the response to specific ligands is species-specific. PXR is also activated by naturally occurring steroids, including pregnenolone and progesterone. It binds to response elements in the promoters of key genes such as CYP3A4 and ABCB1/MDR1. Operating in association with RXR, PXR forms heterodimers and interacts with coactivator NCOA1. Moreover, PXR engages in ligand-dependent interactions with the clock proteins CRY1 and CRY2 through its NR LBD domain, adding another layer of complexity to its regulatory functions.

Human

E. coli

N-6*His

O75469 (S130-S434)

8856

39.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, pH 7.5, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.