R-Cadherin/CDH4 Protein, Human (HEK293, His)

The R-Cadherin/CDH4 protein facilitates cell adhesion through homophilic interactions, aiding in cell connections and potential cell type sorting. It may play a crucial role in retinal development, indicating its significance in orchestrating cellular processes within the context of eye development and function. R-Cadherin/CDH4 Protein, Human (HEK293, His) is the recombinant human-derived R-Cadherin/CDH4 protein, expressed by HEK293 , with C-His labeled tag. The total length of R-Cadherin/CDH4 Protein, Human (HEK293, His) is 714 a.a., with molecular weight of 90-100 kDa.

R-Cadherin, or CDH4, is a calcium-dependent cell adhesion protein that facilitates cellular interactions through homophilic binding, primarily interacting with itself. This characteristic enables cadherins to play a crucial role in the cohesion of connecting cells, potentially contributing to the sorting of diverse cell types. The significance of R-Cadherin extends to retinal development, where its involvement suggests a pivotal function in the intricate processes that govern the formation and organization of the retina. By participating in calcium-dependent cell adhesion, R-Cadherin emerges as a key player in the regulation of cellular arrangements and tissue development, particularly in the context of retinal tissue.

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 0.3577 μg/mL, corresponding to a specific activity is 2.796×10⁴ units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ for this effect is 0.3577 μg/mL , corresponding to a specific activity is 2.796×10⁴ units/mg.

Human

HEK293

C-His

P55283 (H21-A734)

1002  [NCBI]

HNEDLTTRETCKAGFSEDDYTALISQNILEGEKLLQVKFSSCVGTKGTQYETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGA

Approximately 90-115 kDa

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  Greater than 85% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 100 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.