RAC1 Protein, Human (sf9, GST)

The RAC1 protein is a plasma membrane-associated small GTPase that dynamically regulates multiple cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. In its active form, RAC1 regulates processes such as secretory function, phagocytosis, cell polarization, neuronal activity, and growth factor-induced membrane ruffle formation. RAC1 Protein, Human (sf9, GST) is the recombinant human-derived RAC1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of RAC1 Protein, Human (sf9, GST) is 189 a.a., with molecular weight of ~44 kDa.

The RAC1 Protein, a plasma membrane-associated small GTPase, dynamically cycles between its active GTP-bound and inactive GDP-bound states, exerting regulatory control over a spectrum of cellular responses. In its active state, RAC1 binds to various effector proteins, modulating processes such as secretory functions, phagocytosis of apoptotic cells, epithelial cell polarization, neuronal adhesion, migration, differentiation, and growth-factor-induced formation of membrane ruffles. It forms an active heterodimer with Rho GDI in the cytosol, known as sigma 1, participating in the stimulation of NADPH oxidase activity in macrophages. Essential for SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, RAC1 also stimulates PKN2 kinase activity. It collaborates with RAB7A to regulate the formation of ruffled borders in osteoclasts and promotes the nuclear shuttling of NR3C2 in podocytes for proper kidney functioning. In neurons, RAC1 is involved in dendritic spine formation, synaptic plasticity, spine morphogenesis, and synapse formation, highlighting its multifaceted role in neurobiology. Furthermore, RAC1 plays a crucial role in regulating GABA(A) receptor synaptic stability and GABAergic inhibitory synaptic transmission through PAK1 activation and F-actin stabilization. The isoform B of RAC1 exhibits distinctive features in its GEF-independent GDP/GTP exchange and impaired GTP hydrolysis, partially restored by GTPase-activating proteins, suggesting nuanced regulatory mechanisms in its effector interactions.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

P63000 (M1-C189)

5879  [NCBI]

Approximately 44 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM GSH, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.