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  4. RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST)

RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST)

Cat. No.: HY-P702733
Handling Instructions Technical Support

RAF1 is a kinase that links Ras GTPases to the MAPK/ERK cascade, influencing cell fate decisions. Activated RAF1 initiates the MAPK cascade and phosphorylates MEK1/2 and ERK1/2. RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST) is the recombinant human-derived RAF1, expressed by Sf9 insect cells , with His, GST labeled tag. ,

For research use only. We do not sell to patients.

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Description

RAF1 is a kinase that links Ras GTPases to the MAPK/ERK cascade, influencing cell fate decisions. Activated RAF1 initiates the MAPK cascade and phosphorylates MEK1/2 and ERK1/2. RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST) is the recombinant human-derived RAF1, expressed by Sf9 insect cells , with His, GST labeled tag. ,

Background

RAF1, a serine/threonine-protein kinase, serves as a pivotal regulatory link connecting membrane-associated Ras GTPases to the MAPK/ERK cascade, playing a decisive role in determining cellular fate decisions such as proliferation, differentiation, apoptosis, survival, and oncogenic transformation. Upon activation, RAF1 initiates a MAPK cascade involving the sequential phosphorylation of dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1, facilitated by PAK1, phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75' and adenylyl cyclases (ADCY2, ADCY5, and ADCY6), activating them. Additionally, RAF1 phosphorylates PPP1R12A, inhibiting its phosphatase activity. Beyond its role in the MAPK cascade, RAF1 can activate NF-kB and inhibit key signaling molecules involved in motility, apoptosis, proliferation, and angiogenesis. It exerts anti-apoptotic effects by translocating to the mitochondria, binding BCL2, and displacing BAD/Bcl2-antagonist of cell death. Furthermore, RAF1 regulates Rho signaling, migration, wound healing, and is implicated in oncogenic transformation by repressing the tight junction protein occludin (OCLN). Notably, RAF1 restrains caspase activation in response to specific stimuli, including Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Species

Human

Source

Sf9 insect cells

Tag

His;GST

Accession

P04049-1 (S306-F648, Y340D,Y341D)

Gene ID

5894

Synonyms
RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1
Purity

Greater than 80 % as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RAF1 Protein, Human (Y340D,Y341D, sf9, His, GST)
Cat. No.:
HY-P702733
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