RBBP4 Protein, Mouse (sf9, His)

The RBBP4 protein is a core histone-binding subunit that directs chromatin regulators and histone deacetylases to their substrates and participates in chromatin metabolism.RBBP4 Protein, Mouse (sf9, His) is the recombinant mouse-derived RBBP4 protein, expressed by Sf9 insect cells , with N-His labeled tag.

RBBP4, as a core histone-binding subunit, plays a pivotal role in orchestrating the assembly and function of various chromatin-regulating complexes. It serves as a crucial mediator, directing chromatin assembly factors, chromatin remodeling factors, and histone deacetylases to their histone substrates, with regulatory nuances dictated by nucleosomal DNA. RBBP4 is integral to multiple complexes that govern chromatin metabolism, including the chromatin assembly factor 1 (CAF-1), core histone deacetylase (HDAC), nucleosome remodeling and histone deacetylase (NuRD), polycomb repressive complex 2 (PRC2), and nucleosome remodeling factor (NURF). In the CAF-1 complex, alongside CHAF1B and CHAF1A, RBBP4 contributes to chromatin assembly post-DNA replication and repair. As part of the HDAC complex, which includes HDAC1, HDAC2, and RBBP7, RBBP4 facilitates histone deacetylation and transcriptional repression. Additionally, in the NuRD complex, PRC2 complex, and NURF complex, RBBP4 engages in collaborative efforts with various subunits to modulate chromatin structure and gene expression. This multifaceted engagement highlights RBBP4's intricate role in epigenetic regulation, impacting diverse cellular processes.

Mouse

Sf9 insect cells

N-His

Q60972 (M1-S425)

19646  [NCBI]

Approximately 50 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.