RelA/NFkB p65 Protein, Human (GST)

RelA/NF-kappa-B p65 is a key transcription factor that mediates inflammatory, immune, and cellular processes in response to multiple stimuli. Inactive in the cytoplasm, it forms a complex with I-kappa-B. RelA/NFkB p65 Protein, Human (GST) is the recombinant human-derived RelA/NFkB p65, expressed by E. coli , with N-GST labeled tag.

RelA/NF-kappaB p65 is a multifaceted transcription factor integral to diverse cellular processes, acting as the endpoint of signal transduction cascades governing inflammation, immunity, differentiation, growth, tumorigenesis, and apoptosis. Functioning within NF-kappaB heterodimeric complexes, RelA pairs predominantly with NFKB1/p50 and REL, exerting transcriptional activation of target genes. This complex is held inactive in the cytoplasm by binding with NF-kappaB inhibitor (I-kappa-B) proteins. Upon stimulation, I-kappa-B is phosphorylated by I-kappa-B kinases, triggering its degradation, liberating the active NF-kappaB complex. RelA demonstrates a weak DNA-binding site, directly participating in DNA binding within the NF-kappaB complex. Beyond direct transcriptional activation, RelA modulates promoter accessibility, indirectly influencing gene expression. Intriguingly, RelA also interacts with a myriad of proteins, including various inhibitors, kinases, deacetylases, and other cofactors, underscoring its role in intricate cellular networks. Furthermore, in the context of microbial infection, RelA plays a key role in regulating the IFN response during SARS-CoV-2 infection and has been implicated in latent HIV-1 transcriptional reactivation.

Human

E. coli

N-GST

Q04206-1 (M1-Y306)

5970  [NCBI]

MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY

Approximately 60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.