RET Protein, Mouse (HEK293, His)

RET protein is an important receptor tyrosine protein kinase that drives a variety of cellular processes such as proliferation, migration, and differentiation when bound to GDNF ligand. It coordinates organogenesis, shapes the enteric nervous system and promotes kidney development. RET Protein, Mouse (HEK293, His) is the recombinant mouse-derived RET protein, expressed by HEK293 , with C-6*His labeled tag and F174S mutation. The total length of RET Protein, Mouse (HEK293, His) is 609 a.a.

RET protein is a receptor tyrosine-protein kinase that plays a crucial role in various cellular processes, including cell proliferation, neuronal navigation, cell migration, and cell differentiation when it binds with glial cell-derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates the balance between cell death and survival, as well as positional information. During intestine organogenesis, RET protein is essential for coordinating molecular mechanisms and is involved in the development of the enteric nervous system and renal organogenesis in embryonic life. Additionally, it promotes the formation of Peyer's patch-like structures, which are vital components of the gut-associated lymphoid tissue. RET protein modulates cell adhesion through its cleavage by caspase in sympathetic neurons and facilitates cell migration in an integrin-dependent manner, involving proteins such as ITGB1 and ITGB3. Furthermore, RET protein is implicated in the development of the neural crest. In the absence of ligand, it exhibits activity that triggers apoptosis via receptor intracellular caspase cleavage. Moreover, RET protein acts as a dependence receptor, promoting survival and downregulating growth hormone production in somatotrophs when the ligand GDNF is present, but inducing apoptosis in the absence of GDNF. It also regulates nociceptor survival and size and triggers the differentiation of rapidly adapting mechanoreceptors. In various diseases, such as neuroendocrine cancers, RET protein acts as a mediator by modulating cell migration regulated by aberrant integrins. Additionally, RET protein interacts with the GDF15-receptor GFRAL in the brainstem to mediate GDF15-induced cell signaling, leading to the inhibition of food intake. Furthermore, RET protein activates the MAPK and AKT signaling pathways.

Measured by its binding ability in a functional ELISA. Immobilized GDNF-GFR alpha-1 complex using 4 ng/mL rmGDNF (HY-P7359) and 1 µg/mL rmGDNFR-alpha-1(HY-P76360), can bind Recombinant biotinylated Mouse RET. The ED₅₀ for this effect is 2.282 μg/mL.

Mouse

HEK293

C-6*His

P35546 (L29-R637, F174S)

19713

LYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQHLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWPGCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSSRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGDSLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTASAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRATMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARRYAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVATDRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPLCDALCR

Approximately 92-125 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.